thr 77 - An Overview
thr 77 - An Overview
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Antibodies certain to the blocking peptide no longer bind to the epitope to the focus on protein. By comparing the staining through the blocked antibody vs . the antibody by itself, you can see which staining is certain.
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to be certain the best possible outcomes are...
This guideline outlines further more probable optimization of cellular and immunoassay parameters to be certain the absolute best results are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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Blocking peptides are peptides that bind particularly towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope regarded from the antibody.
Focus on Information and facts Cyclin E belongs for the really conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which contribute for the temporal coordination of each mitotic occasion. Cyclin E types a posh with and functions like a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary and it is degraded as cells development by S stage.
Overexpression of this gene has been noticed in lots of tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was found to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential job in advertising and marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two extra splice variants were being reported but detailed nucleotide sequence details is just not nonetheless obtainable.
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